Hello All.
I hope you are ready for class tomorrow.
We will be having a local scientific visitor: Dr. Alia, Solid State MRI Group. She will present in the first half of the class on the research being conducted in her group, and provide us with a tour of the facilities.
The second half of the class will be dedicated to group discussion - about the class, what you have experienced thus far, the course homework, and the final project. I encourage you all to bring your thoughts, questions, concerns and even critiques to the discussion! If we have time, i will also show you some of my work, as requested.
Otherwise, don't forget to complete this weeks readings (see below).
Jennifer.
Singer, Peter. “All Animals are Equal… or why supporters of liberation for blacks and women should support animal liberation too.” In Animal Liberation. Norfolk: Lowe and Brydone Ltd, 1976. pp. 1- 26
SAMIRA KABLI, A. ALIA, HERMAN P. SPAINK, FONS J. VERBEEK, and HUUB J.M. DE GROOT
Magnetic Resonance Microscopy of the Adult Zebrafish
ZEBRAFISH Volume 3, Number 4, 2006 © Mary Ann Liebert, Inc.
Niels Braakman, MS, Jo ̈ rg Matysik, PhD, Sjoerd G. van Duinen, MD, PhD, Fons Verbeek, PhD, Reinhard Schliebs, PhD, Huub J.M. de Groot, PhD, and A. Alia, PhD.
Longitudinal Assessment of Alzheimer’s �-Amyloid Plaque Development in Transgenic Mice Monitored by In Vivo Magnetic Resonance Microimaging
JOURNAL OF MAGNETIC RESONANCE IMAGING 24:530 –536 (2006)
Thursday, April 17, 2008
Monday, April 14, 2008
Liver Cells - update and next class.
Report from the lab:
I hope you all enjoyed last week's class as much as I did! It was an excellent / complex / visceral procedure – that allowed us all to inherently grasp the bodyness and the food chain of the lambs livers – and their extrapolated existence as cells under the microscope and in the dish. I look forward to your comments and reflections during next class – and I am very curious to hear more from the objectors as well.
A resounding success – and a complete failure, where sterility is concerned. A number of you were able to isolate living cells, but pretty much every dish was contaminated with bacteria. You’re a dirty bunch! (just kidding) I went into the university to feed the cells on Sunday, and found one dish in particular that was hosting large fungal colonies – the rest were getting cloudy with bacteria. I had planned on keeping them alive in time for next week – but quite frankly I was worried about contaminating the incubator. So, I am sorry to report that all of the cells have been disposed off. They were packed in a biohazard bag, and left for autoclaving. Life is a violent process, even in the lab.
Other news…
I have a little homework assignment for everyone. On April 25 we will be doing a genetic modification of bacteria protocol. This process usually takes place in petri dishes (like we used in the first class.) However, we thought it may be interesting to use some alternative vessels. I am asking that you all bring in small vessels for next class (April 18th) that we can use for this lab. They must be small, made of a material that can be heat sterilized in an oven - and think of interesting shapes - transparencies - alternative uses.
I hope you all enjoyed last week's class as much as I did! It was an excellent / complex / visceral procedure – that allowed us all to inherently grasp the bodyness and the food chain of the lambs livers – and their extrapolated existence as cells under the microscope and in the dish. I look forward to your comments and reflections during next class – and I am very curious to hear more from the objectors as well.
A resounding success – and a complete failure, where sterility is concerned. A number of you were able to isolate living cells, but pretty much every dish was contaminated with bacteria. You’re a dirty bunch! (just kidding) I went into the university to feed the cells on Sunday, and found one dish in particular that was hosting large fungal colonies – the rest were getting cloudy with bacteria. I had planned on keeping them alive in time for next week – but quite frankly I was worried about contaminating the incubator. So, I am sorry to report that all of the cells have been disposed off. They were packed in a biohazard bag, and left for autoclaving. Life is a violent process, even in the lab.
Other news…
I have a little homework assignment for everyone. On April 25 we will be doing a genetic modification of bacteria protocol. This process usually takes place in petri dishes (like we used in the first class.) However, we thought it may be interesting to use some alternative vessels. I am asking that you all bring in small vessels for next class (April 18th) that we can use for this lab. They must be small, made of a material that can be heat sterilized in an oven - and think of interesting shapes - transparencies - alternative uses.
Primary Isolation of Lamb Liver Cells
Anne Kienhuis | Jennifer Willet
2008
Materials:
1 lamb liver within 48 hours of slaughter
1 cooler w/ ice
1 cutting board
4 syringes – w/ terumo cannula
1 plastic tray
1 plastic stand w/ holes
2 beakers
1 knife
1 tweezers
1 filter + 100 mesh
Pipette Gun
10 ml pipettes
25 ml pipettes
6 well tissue culture tray
70% ethanol
PBS
HBSS 10% solution w/ sterile water
DMEM + Glutamax + Collagenase (Add 250 mg Collagenase to 500 mL DMEM)
DMEM + Glutamax + Penstrep +10% FBS
DMEM + Glutamax + Penstrep
1) Put Liver on ice immediately after purchase, and transport to laboratory
2) Sterilize all equipment with 70% ethanol
3) Immediately (before the blood has much time to clot) slice a small piece of lobe off with a single clean cut.
4) Place liver on top of perforated stand – on top of a beaker - within a plastic tray.
5) Using a syringe and tc – perfuse exposed veins with PBS until color is drained from liver.
6) Choose three prominent vein entries into the liver (test their prominence by injecting HBSS solution into veins.
7) place three stationary syringes + tc into these openings, and crazy glue into place
8) Seal entire cut opening of liver lobe with the application of crazy glue
9) Simultaneously flush each opening with 100ml of HBSS solution SLOWLY.
10) Take a new beaker.
11) Simultaneously flush each opening with 100ml of DMEM w/ Collagenase solution SLOWLY – ensuring that all excess is captured by the beaker below.
12) Repeat three times
13) Remove plugs, and crazy glue carefully with tweezers
14) Take a moment to clean out the beaker with a towel and 70% ethanol (take care that ethanol is evaporated before use!)
15) Make several cuts into the liver.
16) Pour DMEM + FBS over cuts in liver – capturing the solution in your beaker. Do this several times. Eventually, stirring the liver gently in the beaker creating a cell suspension solution.
17) Filter into a clean beaker twice, once with the filter alone, and the second time with the 100 mesh nestled within the filter.
18) Pour final solution back into the DMEM + FBS 50ml vial.
19) Centrifuge for 5 min at 75g.
20) Remove excess DMEM with a pipette gun, and take off top layer of red blood cells and dead cells.
21) Add 10 ml of DMEM + FBS, pipetteing up and down stirring cells.
22) (it is good at this point to repeat steps 19 – to 21.
23) Place solution evenly in 6 well TC tray
24) Rotate tray in a figure 8 pattern evenly distributing the cells over the tray, and incubate at 37 C w/ CO2 5%.
2008
Materials:
1 lamb liver within 48 hours of slaughter
1 cooler w/ ice
1 cutting board
4 syringes – w/ terumo cannula
1 plastic tray
1 plastic stand w/ holes
2 beakers
1 knife
1 tweezers
1 filter + 100 mesh
Pipette Gun
10 ml pipettes
25 ml pipettes
6 well tissue culture tray
70% ethanol
PBS
HBSS 10% solution w/ sterile water
DMEM + Glutamax + Collagenase (Add 250 mg Collagenase to 500 mL DMEM)
DMEM + Glutamax + Penstrep +10% FBS
DMEM + Glutamax + Penstrep
1) Put Liver on ice immediately after purchase, and transport to laboratory
2) Sterilize all equipment with 70% ethanol
3) Immediately (before the blood has much time to clot) slice a small piece of lobe off with a single clean cut.
4) Place liver on top of perforated stand – on top of a beaker - within a plastic tray.
5) Using a syringe and tc – perfuse exposed veins with PBS until color is drained from liver.
6) Choose three prominent vein entries into the liver (test their prominence by injecting HBSS solution into veins.
7) place three stationary syringes + tc into these openings, and crazy glue into place
8) Seal entire cut opening of liver lobe with the application of crazy glue
9) Simultaneously flush each opening with 100ml of HBSS solution SLOWLY.
10) Take a new beaker.
11) Simultaneously flush each opening with 100ml of DMEM w/ Collagenase solution SLOWLY – ensuring that all excess is captured by the beaker below.
12) Repeat three times
13) Remove plugs, and crazy glue carefully with tweezers
14) Take a moment to clean out the beaker with a towel and 70% ethanol (take care that ethanol is evaporated before use!)
15) Make several cuts into the liver.
16) Pour DMEM + FBS over cuts in liver – capturing the solution in your beaker. Do this several times. Eventually, stirring the liver gently in the beaker creating a cell suspension solution.
17) Filter into a clean beaker twice, once with the filter alone, and the second time with the 100 mesh nestled within the filter.
18) Pour final solution back into the DMEM + FBS 50ml vial.
19) Centrifuge for 5 min at 75g.
20) Remove excess DMEM with a pipette gun, and take off top layer of red blood cells and dead cells.
21) Add 10 ml of DMEM + FBS, pipetteing up and down stirring cells.
22) (it is good at this point to repeat steps 19 – to 21.
23) Place solution evenly in 6 well TC tray
24) Rotate tray in a figure 8 pattern evenly distributing the cells over the tray, and incubate at 37 C w/ CO2 5%.
Bacteria Cultures: one week later....
Bacteria Samples in the Incubator... Did one of you pee in your dish?
Monday, April 7, 2008
Class Prep: 02 Tissue Culture
Hello All.
I am very pleased with our work last class. I feel we had a good introduction to BioArt in general, and a nice simple laboratory introduction as well.
We will have a visitor next week: Marie Pier Boucher. She is a PhD student from The University of Exeter. She will give a presentation: Transductive aesthetics: Bioart’s Ethics and Politics.
For next week, please do the following two readings:
Jens Hauser. "Bio Art – Taxonomy of an Etymological Monster" from Hybrid living in paradox: Ars Electronica 2005. Christine Schöpf, Gerfried Stocker. Edts. Ostfildern-Ruit, New York, Hatje Cantz: D.A.P. Distributed Art Publishers, 2005. p.182.
Catts, Oron, and Ionat Zurr. "Are the Semi-Living semi-good or semi-evil?" in Technoetic arts: an international journal of speculative research. Issue 1, 2003
They are available online at: (same as every week)
www.jenniferwillet.com/readings
I will also post my powerpoint lecture from last week for anyone who would like a copy.
We will be starting at 1:15 every week to allow students to arrive form morning classes. On Friday we will be starting in the lab first. So please meet me in the front lobby at 1:15 sharp - and we will go to the lab together.
Otherwise, students who want to see their bacteria from last week under the microscope can meet at 11:00 am (also in the lobby) and I will take you in. If you ever get stuck locked out - you can always ask at the front desk for Anne Kinehuis - or myself (in Liesbeth van der Velden's office). And they will help you.
See you on Friday!
Jennifer.
I am very pleased with our work last class. I feel we had a good introduction to BioArt in general, and a nice simple laboratory introduction as well.
We will have a visitor next week: Marie Pier Boucher. She is a PhD student from The University of Exeter. She will give a presentation: Transductive aesthetics: Bioart’s Ethics and Politics.
For next week, please do the following two readings:
Jens Hauser. "Bio Art – Taxonomy of an Etymological Monster" from Hybrid living in paradox: Ars Electronica 2005. Christine Schöpf, Gerfried Stocker. Edts. Ostfildern-Ruit, New York, Hatje Cantz: D.A.P. Distributed Art Publishers, 2005. p.182.
Catts, Oron, and Ionat Zurr. "Are the Semi-Living semi-good or semi-evil?" in Technoetic arts: an international journal of speculative research. Issue 1, 2003
They are available online at: (same as every week)
www.jenniferwillet.com/readings
I will also post my powerpoint lecture from last week for anyone who would like a copy.
We will be starting at 1:15 every week to allow students to arrive form morning classes. On Friday we will be starting in the lab first. So please meet me in the front lobby at 1:15 sharp - and we will go to the lab together.
Otherwise, students who want to see their bacteria from last week under the microscope can meet at 11:00 am (also in the lobby) and I will take you in. If you ever get stuck locked out - you can always ask at the front desk for Anne Kinehuis - or myself (in Liesbeth van der Velden's office). And they will help you.
See you on Friday!
Jennifer.
Class 01: Bacteria Cultures
or how dirty are scientists anyways......
Gorlaeus Laboratories
workstation
students
Gorlaeus Laboratories
workstation
students
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